Rate of Activation and Electrophoretic Mobility of Unmodified and Partially Degraded Plasminogen Effects of 6-Aminohexanoic Acid and Related Compounds

Abstract

Pretreatment of native plasminogen (plg) with plasmin or activators resulted in an increase in the rate of activation (RA) by urokinase and a shift in mobility from β₂ to γ in agarose gel electrophoresis at pH 8.6. 6-Aminohexanoic acid (6-AHA) had the same effects at c=10^-4 to 3.3 ± 10^-3 mol/1, probably owing to a reversible binding to pig. 6-AHA produced a decrease in RA at higher c, probably owing to a reversible binding to urokinase. The high RA of pretreated pig was inhibited at c > 10^-4 mol/1. L-Lysine and trans-4-aminomethylcyclohexane-1-carboxylic acid had the same effects as 6-AHA on RA and mobility at 7 times higher and lower c. Dodecyl sulphate polyacrylamide electrophoresis showed that pretreated pig had a lower Mr than native plg (δMr 3700).

Key Words:

• Activation rate
• 6-aminohexanoic acid
• electrophoretic mobility
• L-lysine
• plasmin
• plasminogen
• tissue activator
• trans-4-aminomethylcyclo-hexane-1-carboxylic acid
• urokinase