77
Views
37
CrossRef citations to date
0
Altmetric
Original Article

Distribution of LDH Isozymes in Human Skeletal Muscle

, , , &
Pages 307-312 | Received 25 Oct 1973, Accepted 15 Mar 1974, Published online: 28 Aug 2009
 

Abstract

LDH isozyme patterns in human skeletal muscle have been studied in 17 subjects of both sexes and varying age groups (16–55 years). Homogenized muscle biopsy materials have been analysed for total LDH activity (Vmax) in both directions, as well as enzyme activity attributable to isozymes. In addition, different isozymes have been electrophoretically separated and stained. In human skeletal muscle all (5) LDH isozymes were present, although the relative contribution from the more heart-specific isozymes (LDH 1 plus 2) declined the higher the total LDH activity. In absolute terms, however, this decline corresponded to a 2 to 3 fold increase in the activity of LDH 1 plus 2 when the two extremes with low and high total LDH activity, respectively, were compared. Per cent LDH 1 plus 2 of total LDH activity was found to increase with an increase in per cent slow-twitch fibres. As an increase in total LDH activity was synonymous to an increase in per cent fast-twitch fibres, it was concluded that LDH isozyme pattern in human skeletal muscle was related to the fibre type. Moreover, the slow-twitch fibre had a relatively higher activity of the isozyme fractions corresponding to the more heart-specific isozymes LDH 1 and LDH 2.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.