Cholesterol Esterification by the Rat Liver Golgi Apparatus

G. Assmann National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland, USA

R. W. Mahley National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland, USA

B. D. Davis National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland, USA

K. S. Holcombe National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland, USA

Abstract

Cholesterol esterification has been measured in rat liver subcellular fractions, using [7–³H] cholesterol, 2–[1–¹⁴C oleoyl]-sn-phosphatidylcholine and [1–¹⁴C] palmitoyl CoA as substrates. The isolated Golgi apparatus had significant fatty acyl CoA-cholesteryl acyltransferase activity with an optimum at pH 5.8. Cholesteryl esters formed in vitro were predominantly associated with Golgi VLDL particles. The results emphasize the importance of the Golgi apparatus in the assembly of lipoproteins.

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Cholesterol Esterification by the Rat Liver Golgi Apparatus

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Cholesterol Esterification by the Rat Liver Golgi Apparatus

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