Plasma Lipoproteins in Familial Lecithin:Cholesterol Acyltransferase Deficiency: Effects of Incubation with Lecithin: Cholesterol Acyltransferase in vitro

Kaare R. Norum Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

John A. Glomset Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

Alex V. Nichols Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

Trudy Forte Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

John J. Albers Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

Weiling C. King Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

Carolyn D. Mitchell Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

Kenneth R. Applegate Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

Elaine L. Gong Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

Veneracion Cabana Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

Egil Gjone Institute of Nutrition Research and Medical Department A, School of Medicine, University of Oslo, Norway; Dept. of Medicine, Regional Primate Research Center and Northwest Lipid Research Clinic, University of Washington, Seattle, Washington, USA; Donner Research Laboratory, University of California, Berkeley, California, USA

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Abstract

To study the effect of lecithin: cholesterol acyltransferase (LCAT) on the plasma lipoproteins of patients with familial LCAT deficiency, whole plasma or the lipoprotein fraction of d < 1.006 g/ml (VLDL) was incubated in the presence of LCAT and subsequently examined by chemical, physical, and immunological techniques. The following occurred upon incubating either hyperlipemia or nonlipemic plasma: The concentrations of polar lipids decreased, particularly in the large molecular weight lipoprotein subfraction of d 1.019-1.063 g/ml (LDL₂) and in the lipoprotein fraction of d 1.063-1.25 g/ml (HDL). The concentration of cholesteryl ester (CE) increased, particularly in the VLDL and in the lipoprotein fractions of d 1.006-1.019 g/ml (LDL₁) and LDL₂. The concentration of arginine-rich apolipoprotein decreased in the HDL and increased in the VLDL and LDLi. The concentrations of the C-apoliproteins appeared to change in the opposite direction. The concentration of apolipoprotein B in the LDL increased concomitantly with an increase in the concentration and flotation rate of the small LDL₂. The concentration of apolipoprotein A-I in the HDL increased; and a major component in the HDL fraction became identical in appearance to normal HDL. Upon incubating a patient's isolated VLDL in the presence of LCAT, lipoproteins with properties similar to normal LDL2 were formed. These experiments show that the LCAT reaction can alter the apolipoprotein content and physical properties as well as the lipid content of the patients' lipoproteins.

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Plasma Lipoproteins in Familial Lecithin:Cholesterol Acyltransferase Deficiency: Effects of Incubation with Lecithin: Cholesterol Acyltransferase in vitro

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Plasma Lipoproteins in Familial Lecithin:Cholesterol Acyltransferase Deficiency: Effects of Incubation with Lecithin: Cholesterol Acyltransferase in vitro

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