Separation of Labelled Iodide, Iodoprotein, Iodotyrosines, and Iodothyronines in Serum from Patients Treated with Iodine-131, Using an Improved Chromatographic Method

Abstract

A method is described for complete separation of iodoprotein, 3-iodotyrosine (MIT), 3,5-diiodotyrosine (DIT), 3,5,3′-triiodo-L-thyronine (T₃), and L-thyroxine (T₄) on a single column run on Sephadex G-25 superfine in alkaline solution. Sera from patients treated with ¹³¹I^- have been analysed by this method after removal of I^- by resin dialysis.

Key Words:

• Iodoprotein
• iodotyrosines
• iodothyronines