Evaluation of Radioimmunological Methods for Assay of Plasma and Urinary Aldosterone

Abstract

Two radioimmunological methods for assay of plasma and urinary aldosterone were carefully evaluated. In the plasma method a radioimmunoassay is preceded by chromatography on a Sephadex LH-20 column. The method for urine includes a preextraction, hydrolysis of the acid-labile conjugates of aldosterone, and a radioimmunoassay. Both methods fulfill the criteria of reliability and are suitable for both routine and demanding research assays. The plasma method, using columns of double length, is also applicable to analysis of aldosterone in plasma of newborn children, and pregnant females and in cord plasma. The concentration of plasma aldosterone in healthy subjects on an ad lib salt diet was 162 ± 93 (S.D.) pmol/1 in the supine position and 312 ± 217 (S.D.) pmol/1 upright. The urinary excretion of aldosterone in healthy subjects was 28.3 ± 16.7 (S.D.) nmol/24 h.

Key Words:

• Aldosterone
• mineralocorticoids
• radioimmunoassay
• steroids