Determination of coagulation factors (II-VII-X) and kaolin partial thromboplastin time by the LKB 8600 Reaction Rate Analyser

Abstract

Testing of a modified Reaction Rate Analyser 8600 in the automatic determination of coagulation time was accomplished with the assays Thrombotest®, Normotest® and Kaolin Partial Thromboplastin Time. Variations in extinctions, from the adding of start reagent until coagulation, were recorded. The coagulation activities, found by the automatic method for the assays Thrombotest® and Normotest®, were compared with a manual method. Thrombotest® showed a good correlation: 0.30 > P > 0.25 (paired t test). Normotest® showed significantly different values by the methods: P<0.001 (paired t test). Kaolin Partial Thromboplastin Time (Activated Partial Thromboplastin Time with kaolin as activator) gave significantly lower values— the difference was 3 sec—compared with a manual method, and the precision was considerably improved.

Key Words:

• Activated Thromboplastin Time
• coagulation factors (II-VII-X)
• coagulometer
• Normotest®
• Reaction Rate Analyser LKB 8600
• Thrombotest®