7
Views
5
CrossRef citations to date
0
Altmetric
Original

Peroxidase activity in human granulocytes, inter- and intra-individual variations

, M.D.
Pages 163-167 | Received 31 Aug 1976, Accepted 15 Dec 1976, Published online: 14 Feb 2011
 

Abstract

A kinetic method for quantitation of peroxidase activity (‘myeloperoxidase’, E.C.1.11.1.7.) in human granulocytes has been evaluated. The enzymic activity was determined at 37d`C, pH = 7.0. Substrates: 2-methyxo-phenol (1.25 mmol/l) and H2O2 (0.1 mmol/1). When applied on granulocytes from solitary blood samples from healthy, normal subjects the detected peroxidase activity varied considerably from one person to another (CV = 40–50%). Sequential determinations of the peroxidase activity in granulocytes from selected healthy, normal subjects demonstrated substantial intra-individual variations. Analysis of variance confirmed that the intra-individual variations (CV = 36%) exceeded all other variance components involved.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.