Simultaneous blank correction in a turbidimetric continuous flow system for immunochemical quantitation of plasma proteins

Abstract

By shortening the reaction time by polymer enhancement, it was possible to introduce a line for automatic blank correction in a turbidimetric continuous flow system. The turbidity resulting from the reaction was measured by a simply constructed photometer, the serum turbidity being subtracted by the reference photoelectric cell and the resulting net signal amplified by means of the recorder's stepwise electronic amplifier. Serum transferrin values obtained by this method correlated well with values obtained by estimating blank in separate runs, and with values given by electroimmune assays.

Key Words:

• automation
• blood proteins
• transferrin
• sample blank