Ion exchange analysis of porphyrins in urine

Abstract

An easy and cheap analysis of total porphyrin in urine is described based on the ion exchange resin Dovex 2x8, 50-100 mesh. Recovery of added porphyrin varied between 65 and 90% for uro- and 70 and 95% for coproporphyrin. The use of the method with internal standard is described and exemplified. Ammoniacal solutions of crystalline free porphyrins were used as standards.

Key Words:

• coproporphyrin
• extinction constants
• quantitative analysis
• recovery experiments
• screening
• uroporphyrin