A comparison between diamine oxidases from human post-heparin blood serum, pregnancy blood serum and amniotic fluid

Abstract

Diamine oxidase (DAO) was purified from blood serum after intravenous heparin injection, from pregnancy blood serum and amniotic fluid. On DEAE-Sephadex, DAO from amniotic fluid as well as from pregnancy blood serum was separated in two active fractions with molecular weights of 245,000 and 485,000, respectively. Post-heparin blood serum contained only one active DAO fraction with a molecular weight of 185,000. The purified enzymes showed similar enzymatic properties.

Key Words:

• amniotic fluid
• histaminase
• post-heparin serum
• pregnancy