Solid phase ELISA for serum ferritin

Abstract

We describe a solid-phase enzyme immunoassay for human serum ferritin, with the use of polyethylene glycol to shorten the reaction. The amount of bound enzyme-antibody conjugate used as a second site antibody is proportional to the ferritin titre in the assay. This method is fast, and the reagents are stable for many months at 4°C. The lowest detectable concentration was 5 μg/l. lnterassay coefficients of variation were 9.3 and 12.2%, respectively, at ferritin concentrations of 100 μg/l and 200 μg/l. The data also show that polyethylene glycol accelerates antigen-antibody complex formation in the solid phase. An assay may be completed in 5 h.

Key Words:

• enzyme
• ferritin
• immunoassay
• polyethylene glycol
• serum