27
Views
33
CrossRef citations to date
0
Altmetric
Original Article

Assay of the major bile acids in serum by isotope dilution-mass spectrometry

&
Pages 163-170 | Received 10 Jul 1982, Accepted 03 Dec 1982, Published online: 17 Mar 2010
 

Abstract

A specific method for determination of cholic, chenodeoxycholic and deoxycholic acid in serum based on deuterium-labelled standards and selected ion monitoring is described. The coefficient of variation of the method as calculated from replicate determinations was in the range 1.4–3.3 % for all three bile acids. Recovery experiments were in accord with the high accuracy expected for this type of assay.

In analyses of sera from 23 healthy subjects, the following concentrations were obtained in the fasting state: cholic acid, 0.29 ± 0.81 μmol/l; chenodeoxycholic acid, 0.18 ± 0.32 μmol/l; deoxycholic acid, 0.77 ± 0.38 μmol/l (mean ± SD).

The method may be suitable as a reference method and in experiments with a high need for accuracy.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.