4
Views
4
CrossRef citations to date
0
Altmetric
Original Article

Bile acids in plasma of patients without kidneys

Pages 603-607 | Received 12 Nov 1982, Accepted 20 Jun 1983, Published online: 17 Mar 2010
 

Abstract

Bile acids were measured in plasma of three non-fasting bilaterally nephrectomized patients in chronic haemodialysis. After separation of the bile acid mixture according to mode of conjugation and cleavage of amide and sulphate ester bonds, the bile acids were analysed as their methylester trimethylsilyl ethers on gas—liquid chromatography—mass spectrometry (GC—MS). The total bile acid concentration was 4.10–13.70 μmol·l−1 (N:<14 μmol·l−1) mostly glycine conjugates. Unconjugated bile acids were found in significant amounts in one patient and in trace amounts in another patient. Chenodeoxycholic acid was the predominant individual bile acid constituting 1.89–6.79 μmol·l−1 (N:<10 μmol·l−1). The oxidoreduction product of this bile acid: ursodeoxycholic acid, was found in all patients, whereas the 7α-dehydroxylation product: lithocholic acid, was absent. 1β-hydroxy-deoxycholic acid was a major constituent in two of the cases. Hyocholic acid, the 6α-hydroxylation product of chenodeoxycholic acid was detected in two cases. Tetrahydroxy bile acids and sulphated bile acids were not found in significant amounts.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.