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Original Article

The effect of temperature on plasma ionized calcium measured in whole blood in vitro

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Pages 709-714 | Received 07 Jun 1983, Accepted 09 Aug 1983, Published online: 17 Mar 2010
 

Abstract

Plasma calcium ion concentration (cCa2+) in samples from 50 healthy volunteers was measured at four temperatures (21, 26, 31 and 37°C) using a calcium electrode based on a neutral carrier ligand. A small negative correlation was found between temperature and cCa2+, the coefficient being −0.0017 mmol·l−1·°C−1 (P<0.001). The significance of this in clinical, physiological and instrumentation fields is discussed.

The effect on measured cCa2+ of overnight storage at 4°C was slight, but did increase the variance of the results. It is suggested that the most accurate results of cCa2+ will be obtained from fresh samples measured at body temperature.

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