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Abstract
A specific and simple radioimmunoassay for the determination of MPV-295, 4(5)-[2-(2.6-dimethylphenyl)-ethyl]imidazole, in human serum is described. The antibodies were produced in rabbits immunized with a conjugate of MPV-295 and bovine thyroglobulin prepared by diazo reaction. They were shown to be specific for MPV-295 tested against several synthetic analogues. MPV-295 was iodinated with the chloramine-T method and l25I-MPV-295 was purified in cation exchange chromatography for use as tracer. The sensitivity of the assay is 2–5 pmol/ml depending on the sample volume. Serum determinations were carried out directly with 20–50 μl serum volumes. This assay has been used to monitor serum levels of MPV-295 in pharmacokinetic studies in healthy and hypertensive subjects.