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Technical Note

Automated procedure for measuring TBARS for in vitro comparison of the effect of antioxidants on tissues

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Pages 83-85 | Received 12 Jun 1992, Accepted 04 Sep 1992, Published online: 29 Mar 2011
 

Abstract

Svensson L, Börjesson, Kull B & Sjöquist P-O. Automated procedure for measuring TBARS for in vitro comparison of the effect of antioxidants on tissues. Scand J Clin Lab Invest 1993; 53: 83-85.

In this study we have evaluated a newly developed simplified automated continuous flow technique for the analysis of thiobarbituric acid-reactive-substances (TBARS). The method has been compared with a manual method for measurements of the potency for inhibition of TBARS formation by antioxidants in isolated renal tissue subjected to hypoxia and reoxygenation. The results presented in this report show that this new automated method is reliable, fast, and inexpensive.

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