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Abstract
Background: The effects of estradiol benzoate (EB) on gastric emptying, gastrointestinal transit and plasma levels of cholecystokinin (CCK) were studied in ovariectomized rats. Methods: Gastrointestinal motility was assessed in rats 15 min after intragastric instillation of a test meal containing charcoal and Na 2 51 CrO 4. Gastric emptying was determined by measuring the amount of radiolabeled chromium contained in the small intestine as a percentage of the initial amount received. Gastrointestinal transit was evaluated by calculating the geometric center of distribution of the radiolabeled marker. Blood samples were collected for E 2 and CCK radioimmunoassay. Results: After treatment of EB (4-25 w g/kg), gastric emptying and gastrointestinal transit were inhibited, whereas plasma concentrations of E 2 and CCK were increased in a dose-dependent manner. The selective CCK A receptor antagonists, devazepide and lorglumide, effectively attenuated the EB-induced inhibition of gastric emptying and gastrointestinal transit. L-365,260, a selective CCK B receptor antagonist, did not alter the EB-induced inhibition of gastric emptying and gastrointestinal transit. Conclusions: The results suggest that EB inhibits gastric emptying and gastrointestinal transit in ovariectomized rats via a mechanism involving CCK stimulation and CCK A receptor activation.