Contrasting Action of IL-12 and IL-18 in the Development of Dextran Sodium Sulphate Colitis in Mice

Abstract

Background: Interleukin (IL)-12 and IL-18 are major interferon (IFN)- &#37 -inducing factors that collaborate with each other. The present study was conducted to determine the distinct roles of IL-12 and IL-18 in the development of dextran sulphate sodium (DSS) colitis in mice. Methods: Colitis was induced in IL-12p35 &#109 / &#109 , IL-18 &#109 / &#109 , IL-18 receptor &#109 / &#109 and control mice with DSS. Clinical and histopathological analysis was conducted using survival rate, weight loss score, diarrhoea score, bloody stool score and histological score. In addition, cytokine production by lamina propria mononuclear cells (LPMCs) was examined using the specific enzyme-linked immunoassay. Results: IL-12p35 &#109 / &#109 mice developed only a mild disease associated with no lethality and few histopathological abnormalities. In contrast, IL-18 &#109 / &#109 and IL-18R &#109 / &#109 mice developed more severe colitis associated with high lethality and more histopathological abnormalities compared with control mice. LPMCs from DSS-fed IL-18 &#109 / &#109 mice produced significantly higher amounts of IFN- &#37 , while LPMCs from DSS-fed IL-12 &#109 / &#109 mice produced lower amounts of IFN- &#37 and tumour necrosis factor (TNF)- &#33 compared with control mice. Conclusion: These results suggest that IL-18 might function with manners different from IL-12 at some pathological conditions in the development of colitis.

• Dss
• Il-12
• Il-18
• Murine Colitis