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Abstract
Background: An in vitro multidrug resistance (MDR) system from a human colonic cancer cell line (SW620‐MDR) has been established. To further study the mechanisms at molecular level and prevention of multidrug resistance in clinical practice, it was demonstrated that the expressions of several apoptosis‐related and cell cycle regulator genes were changed in the cells. Methods: A multidrug‐resistant colonic cell line (SW620‐MDR) was established, and the Atlas human cDNA expression array was used for studying the pattern of gene expression in this cell line. Furthermore, Northern hybridization or real‐time PCR analysis confirmed the pattern of gene expression. Results: In the SW620‐MDR cell line the pro‐apoptosis genes, CASP4, BIK, PDCD2, and TACE were expressed with decreased levels, and the anti‐apoptosis genes CD27‐L and IGFBP2 were over‐expressed. Furthermore, the cell cycle regulator genes such as CDK6, CCND1, CDC27HS, CDC16HS, Wee1Hu, MAPKK1, and IGFBP6 were expressed with decreased levels in the drug‐resistant cell line. Conclusions: It is worthwhile investigating whether the differentially expressed pattern of the aforementioned genes exists in the drug‐resistant cancer specimens, and to further understand their functions in the cancer drug‐resistance mechanism.