Functional polymorphism of the NFKB1 gene promoter is not relevant in predisposition to celiac disease

Abstract

Objective. The nuclear factor (NF)-κB is one of the pivotal regulators of autoimmunity and inflammation, which has been shown to be activated in the inflamed mucosa of patients with celiac disease (CD). Recently, in the NFKB1 gene promoter region, a common insertion/deletion (−94ins/delATTG) polymorphism located between two putative key promoter regulatory elements was described. The aim of this study was to investigate the contribution of the −94ins/delATTG NFKB1 gene promoter functional variant to CD genetic predisposition. Material and methods. A case-control cohort comprising 478 patients with CD and 711 healthy controls as well as a panel of 196 celiac families was genotyped for the 94ins/delATTG NFKB1 polymorphism, using a polymerase chain reaction (PCR) method combined with fluorescence technology. Results. We found no statistically significant differences between CD patients and controls when the −94ins/delATTG genotype and allele distributions were compared. Accordingly, the familial analysis did not reach statistically significant deviation in the transmission of −94ins/delATTG alleles to the affected offspring. Conclusions. From these results, it could be suggested that the −94ins/delATTG NFKB1 polymorphism does not play a major role in CD susceptibility.

• Celiac disease
• NFKB1 gene
• polymorphism
• transmission disequilibrium test