Carboxyl Ester Hydrolase and Lipase of Human Pancreatic Juice and Intestinal Content. Behaviour in Gel Filtration

Abstract

Erlanson, Charlotte & Borgström, B. 1970. Carboxyl Ester Hydrolase and Lipase of Human Pancreatic Juice and Intestinal Content. Behaviour in Gel Filtration. Scand. J. Gastroent. 5, 395-400.

Human pancreatic juice contains besides lipase, a carboxylester hydrolase catalysing the hydrolysis of esters with short and long chain fatty acids including cholesterol esters. Calculated from gelfiltration the molecular weight of this enzyme is 300,000, about 4 times as big as a corresponding enzyme from rat. On gel filtration of human intestinal content an aggregation of carboxylesterasc and lipase occurs to a macromolecular form. These aggregates, which are probably formed by hydrophobic interaction, include fatty acids and arc dissociated in bile salt solution. The macromolecular aggregates can be produced by adding pancreatic juice protein to a micellar solution of oleic acid in bile salt followed by gel filtration. The macromolecular complex described probably has no physiological importance.

Key-words:

• Carboxyl ester hydrolase
• fatty acids
• human
• intestinal content
• lipase
• macromolecular aggregates
• pancreatic juicc
• pig
• rat