Experimental Pancreatitis in the Dog. Demonstration of Trypsin in Ascitic Fluid, Lymph and Plasma

Abstract

Ohlsson, K. & Tegner, H. 1973. Experimental Pancreatitis in the Dog. Demonstration of Trypsin in Ascitic Fluid, Lymph, and Plasma. Scand. J. Gastroent. 8, 129-133.

A quantitative immunoassay for dog trypsinogen and trypsin was devised and used for analysis of ascitic fluid, lymph, and plasma from dogs during development of pancreatitis produced by creating a closed duodenal loop. The changes in concentration and electrophoretic homogeneity of trypsin and the protease inhibitors in the body fluids were followed immunochemically. Release of large amounts of trypsinogen was demonstrated first in the ascitic fluid and later in lymph and plasma, a-macroglobulin complexes with trypsin-like activity appeared early in the ascitic fluid and later trypsinai-antitrypsin complexes. The binding capacity of the ct-macroglobulins was finally exhausted. Then all of the trypsinogen of the ascitic fluid was rapidly activated and was complexed by ai-antitrypsin. On saturation of the a-macroglobulins the animals went into shock and died.

Key-words::

• Ascitic fluid
• Chymotrypsin
• lymph
• lymph nodes
• pancreatitis
• reticuloendothelial system
• trypsin