Quantitative Determination of Bile Acids and Their Conjugates Using Thin-layer Chromatography and a Purified 3α-Hydroxysteroid Dehydrogenase

Abstract

Fausa, O. & Skålhegg, B. A. Quantitative determination of bile acids and their conjugates using thin-layer chromatography and a purified 3α-hydroxy-steroid dehydrogenase. Scand. J. Gastroent. 1974, 9, 249-254.

A method is described for the quantitative determination of bile acids in biological material. It is based on the specific enzymatic conversion of the 3α-hydroxyl group of the steroid molecule into a 3-keto-group, using a purified 3α-hydroxysteroid dehydrogenase and spectrophotometric determination of NADH. The individual major bile acids are measured after separation by thin-layer chromatography. The procedure of quantitation is specific, simple, rapid, and reliable.

Key-words::

• Bile
• bile acids
• duodenal aspirates
• 3α-hydroxysteroid dehydrogenase
• thin-layer chromatography