Demonstration of the Abnormal Lipoprotein of Cholestasis, LP-X, by Precipitation with Polyanion

Abstract

Ritland, S. Demonstration of the abnormal lipoprotein of cholestasis, LP-X by precipitation with polyanion. Scand. J. Gastroent. 1974, 9, 507–510.

One hundred serum or plasma samples from patients with manifest or suspected liver disease or familial LCAT deficiency were examined in duplicate for LP-X. After electrophoretic separation in agar LP-X was tested by precipitation with anti-LP-X in one of the duplicates and by precipitation with polyanion in the other. In 60 samples no LP-X could be detected by either method, in 40 samples LP-X was detected by immunoprécipitation, whereas 39 samples were LP-X positive by polyanion-precipitation. in serial dilution with quantitation of LP-X, both methods for demonstration of LP-X showed equal sensitivity and were able to detect concentrations of LP-X as low as 3.3 mg/100 ml. Demonstration of LP-X by precipitation with polyanions seems to be a reliable test that deserves wider distribution as a routine analysis in patients with liver disorders.

Key-words::

• Abnormal lipoprotein of cholestasis
• familial LCAT deficiency
• immunoprecipitation
• LP-X
• polyanion-precipitation