Appearance of Gastrin in Perfusates from the Isolated Gastric Antrum of Dogs

Abstract

Andersson, S. & G. Nilsson. Appearance of gastrin in perfusates from the isolated gastric antrum of dogs. Scand. J. Gastroent. 1974, 9, 619–621.

Three dogs were provided with fully innervated antral pouches and vagally denervated pouches of the fundic stomach. Gastric secretion was stimulated by perfusing the antral pouches with solutions of ethanol of pH 7 or pH 1 or by a test meal with simultaneous perfusion of the antral pouches with buffer solutions of pH 7 or pH 1. The ability of the antral perfusates to induce acid secretion was tested on Heidenhain pouch dogs during background stimulation with urecholine. The presence of gastrin-like immunoreactivity in the antral perfusates was determined by radioimmunoassay. Intravenous administration of extracts from the acid, but not from the neutral, perfusates evoked secretion from the gastric pouches. Gastrin-like immunoreactivity was present in both neutral and acid antral perfusates, but the concentrations and total amounts of gastrin-like activity were approximately 7 times higher in acid perfusates. The results show that there is a ‘leakage’ of gastrin-like activity into the antral lumen that is greatly increased at low pHs. This mechanism may be responsible for the reduction of plasma gastrin levels seen during antral acidification on vagal or local stimulation of gastrin release from the antral mucosa.

Key-words::

• Gastrin release
• inhibition
• radioimmunoassay