Glucose-Induced Insulin Secretion and Pattern of Exocrine Pancreatic Enzymes in the Rat Following Oral and Parenteral Trypsin Inhibitor Administration

Abstract

Ihse, I., Arnesjö, B. & Lundquist, I. Glucose-induced insulin secretion and pattern of exocrine pancreatic enzymes in the rat following oral and parenteral trypsin inhibitor administration. Scand. J. Gastroent. 1974, 9, 719–724.

One daily dose of bovine lung trypsin inhibitor was administered orally or intraperitoneally to rats for 3 weeks. After oral administration the wet weight and the protein content of pancreas were increased. The pancreatic trypsinogen increased in a more pronounced way than the amylase and lipase activities. The trypsin-inhibitor-treated group showed a decreased insulin secretory response 3 and 7 minutes after intravenous glucose administration and 30 and 60 minutes after peroral ingestion of a 30% glucose solution. The glucose tolerance was normal or even increased. No changes in pancreatic wet weight, protein content, enzyme pattern, and endocrine pancreatic function were found after intraperitoneal trypsin inhibitor administration. The results support the hypothesis that the effects on exocrine and endocrine rat pancreatic functions of trypsin inhibitor treatment require the mediation of the gastrointestinal tract.

Key-words::

• Enzyme inhibition
• glucose
• insulin
• pancreas, endocrine
• pancreas, exocrine
• rats
• trypsin