The Effect of Duodenal Juice on the Intrinsic Factor (IF) Activity in Gastric Juice

Abstract

Vatn, M. H. The effect of duodenal juice on the intrinsic factor (IF) activity in gastric juice. Scand. J. Gastroent. 1975, 10, 49-59.

Duodenal juice, collected during basal conditions and during stimulation by pentagastrin. secretin, or cholecystokinin, was incubated with neutralized normal gastric juice. High concentrations of bile or proteolytic activity were accompanied by decrease of IF concentration. The inhibition was time- and temperature-dependent, increased significantly at low pH values, and seemed to be partly due to competitive inhibition by unspecific vitamin Bi2-binding activity. The results are of importance for measurements of IF concentration in gastric juice. Scand. J. Gastroent. 1975, 10, 49-53.

Key-words::

• Bile: bilirubin
• cholecystokinin
• duodenal juice
• gastric juice
• hydrogen ion concentration
• intrinsic factor
• pentagastrin
• proteolytic activity
• secretin
• vitamin B₁₂