Radioimmunoassay of Cationic Trypsin-Like Immunoreactivity in Man

J. FlorholmenLaboratory of Gastroenterology, Dept. of Medicine, and Dept. of Pediatrics, University Hospital of Tromsø, and Institute of Medical Biology, University of Tromsø, Tromsø, Norway

R. JordeLaboratory of Gastroenterology, Dept. of Medicine, and Dept. of Pediatrics, University Hospital of Tromsø, and Institute of Medical Biology, University of Tromsø, Tromsø, Norway

R. L. OlsenLaboratory of Gastroenterology, Dept. of Medicine, and Dept. of Pediatrics, University Hospital of Tromsø, and Institute of Medical Biology, University of Tromsø, Tromsø, Norway

S. KolmannskogLaboratory of Gastroenterology, Dept. of Medicine, and Dept. of Pediatrics, University Hospital of Tromsø, and Institute of Medical Biology, University of Tromsø, Tromsø, Norway

P. G. BurholLaboratory of Gastroenterology, Dept. of Medicine, and Dept. of Pediatrics, University Hospital of Tromsø, and Institute of Medical Biology, University of Tromsø, Tromsø, Norway

Abstract

The present paper describes the preparation of human cationic trypsin and of a stable and fully immunoreactive ¹²⁵I-labelled tosyl-lysine chloromethyl ketone (TLCK) cationic trypsin by a modified chloramine-T method with a high specific radioactivity; the production of an avid and specific rabbit cationic trypsin antiserum; and a sensitive, precise, and specific radioimmunoassay method enabling measurements of fasting serum trypsin-like immunoreactivity (CTLI) in the low ng/ml range in normals; the significant rise in serum CTLI in patients with normal pancreatic exocrine secretion; and the absence of any rise in patients with severely reduced pancreatic exocrine secretion after intravenous injection of secretin; a markedly elevated fasting serum CTLI level in patients with acute pancreatitis; fasting CTLI in duodenal juice; and separation of CTLI in one minor and one major molecular component in fasting serum.

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Radioimmunoassay of Cationic Trypsin-Like Immunoreactivity in Man

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Radioimmunoassay of Cationic Trypsin-Like Immunoreactivity in Man

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