Distribution of mucosal PD-1 expressing T cells in patients with colitis of different etiologies

Abstract

Background

Immunotherapy, targeting programmed death-1 (PD-1) enhances antitumor T-cell activity in patients with malignancies. Blocking PD-1 or its ligand may lead to fulminant colitis as serious adverse event in these patients. Since little is known of the presence and role of PD-1⁺T cells in colitis of different etiologies, we determined PD-1⁺T cells in mucosal specimens of patients with inflammatory bowel disease, infectious colitis (InfC), immunotherapy-related colitis (ImC) and healthy controls (HC).

Methods

Newly diagnosed patients with ulcerative colitis (UC, n = 73), Crohn’s disease (CD, n = 50), InfC (n = 5), ImC (n = 8) and HC (n = 8) were included. Baseline inflamed colonic biopsies were studied with immunohistochemistry and flowcytometry.

Results

Using immunohistochemistry, PD-1 was not present on lymphocytes in the epithelium of all patients, nor in HC. The percentage PD-1⁺ of all lymphocytes in the lamina propria was 40% in UC, 5% in InfC, 3% in ImC and 0% in HC. Flowcytometry showed significant higher percentages of PD-1⁺T cells in inflamed biopsy specimens of UC patients (22%) compared to all other groups: CD patients (13%), InfC (12%), ImC (5%) and HC (6%).

Conclusion

There are relevant differences in distribution and frequencies of mucosal PD-1⁺ T-cell subsets in patients with UC, CD, InfC and ImC, supporting the hypothesis that these types of colitis are driven by different immunological pathways. The increased numbers of PD-1⁺ and PD-L1⁺ lymphocytes in the colonic mucosa of UC patients suggest that the PD-1/PD-L1 pathway might be more activated in UC than in CD.

Keywords:

• IBD
• PD-L1
• therapy
• T cells
• immunotherapy-related colitis

Disclosure statement

The authors declare no conflict of interest.