Evaluation of an automated extraction method for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Cobas Amplicor PCR from different sample materials

Ralf Geertsen From the Institute Virion Ltd., Molecular Biology, Rueschlikon, SwitzerlandCorrespondence[email protected]

Paul Friderich Medica, Medizinische Laboratorien Dr. F. Kaeppeli AG, Zürich, Switzerland

Marinko Dobec From the Institute Virion Ltd., Molecular Biology, Rueschlikon, Switzerland; Medica, Medizinische Laboratorien Dr. F. Kaeppeli AG, Zürich, Switzerland

Stefan Emler Smartgene GmbH, Zug, Switzerland

Abstract

A commercially available automated device (MagNA Pure LC™) was adapted for nucleic acid extraction of urogenital specimen for subsequent PCR detection of Chlamydia trachomatis and Neisseria gonorrhoeae in a clinical laboratory. Results were compared to the standard manual extraction procedure and showed excellent correlation, with even slightly increased sensitivity.

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Evaluation of an automated extraction method for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Cobas Amplicor PCR from different sample materials

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Evaluation of an automated extraction method for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Cobas Amplicor PCR from different sample materials

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