Evaluation of phenotypic and molecular methods for screening and detection of methicillin-resistant Staphylococcus aureus

Anne-Marie Kerttula From the Hospital Bacteria Laboratory, Department of Bacteriology and Inflammatory Diseases, National Public Health Institute (KTL), Helsinki, FinlandCorrespondence[email protected]

Sointu Mero From the Hospital Bacteria Laboratory, Department of Bacteriology and Inflammatory Diseases, National Public Health Institute (KTL), Helsinki, Finland

Tanja Pasanen From the Hospital Bacteria Laboratory, Department of Bacteriology and Inflammatory Diseases, National Public Health Institute (KTL), Helsinki, Finland

Jaana Vuopio-Varkila From the Hospital Bacteria Laboratory, Department of Bacteriology and Inflammatory Diseases, National Public Health Institute (KTL), Helsinki, Finland

Anni Virolainen From the Hospital Bacteria Laboratory, Department of Bacteriology and Inflammatory Diseases, National Public Health Institute (KTL), Helsinki, Finland

Abstract

An in-house PCR was compared with the GenoType® MRSA and the MRSA EVIGENE^TM tests, both of which corresponded 100% with the results of in-house PCR. The cefoxitin disk diffusion method was superior to both the oxacillin disk diffusion and minimum inhibitory concentration tests for predicting the mecA status.

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Evaluation of phenotypic and molecular methods for screening and detection of methicillin-resistant Staphylococcus aureus

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Evaluation of phenotypic and molecular methods for screening and detection of methicillin-resistant Staphylococcus aureus

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