Serodiagnosis of Viral Hepatitis A by a Solid-phase Radioimmunoassay Specific for IgM Antibodies

Bengt G. HanssonFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Jean K. CalhounFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Doris C. WongFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Stephen M. FeinstoneFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Robert H. PurcellFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Claudio S. PannutiFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Joao L. PereiraFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Raymond S. KoffFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Jules L. DienstagFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

Sten IwarsonFrom the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, the Gl Unit, Massachusetts General Hospital, and the Hepatology Section, VA Medical Center, Boston, USA, The Hospital Do Servido Publico Est Adual, Sao Paulo, Brazil, Departments of Clinical Virology, University of Lund, General Hospital, Malmö and Infectious Diseases, University of Göteborg, East Hospital, Göteborg, Sweden,

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Abstract

A solid-phase radioimmunoassay for detecting specific IgM antibodies to hepatitis A virus (HAV) was developed and characterized. The test utilizes microtiter plates coated with anti-IgM to specifically absorb the IgM antibodies from the test serum. The anti-hepatitis A IgM antibodies are measured by the specific consecutive binding of hepatitis A antigen and radiolabeled anti-hepatitis A antibodies (anti-HA). In 6 chimpanzees infected with HAV, IgM anti-HA was detected from about the first date of elevated transaminases and was positive for about 3 months. The usefulness of the test was confirmed by testing acute phase sera of 30 patients from a common source outbreak of epidemic hepatitis, and negative sera from 2 control groups. A collection of serum specimens from 190 patients with sporadic HBsAg-negative hepatitis in Brazil was also tested and an etiologic association with HAV was confirmed in the majority of these cases.

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Serodiagnosis of Viral Hepatitis A by a Solid-phase Radioimmunoassay Specific for IgM Antibodies

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Serodiagnosis of Viral Hepatitis A by a Solid-phase Radioimmunoassay Specific for IgM Antibodies

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