Integrality of Benign Prostatic Hyperplasia Tissues After Transurethral Thermotherapy Evidenced by Transmittance Fourier Transform Infrared Spectroscopy Combined with Thermal Analyzer

Abstract

Transmittance Fourier transform infrared microspectroscopy combined with differential scanning calorimetry was used to simulate the clinically transurethral thermotherapy of benign prostatic hyperplasia (BPH) by in vitro isothermal determination. The simulative study to the thermotherapy of epithelium or stroma in BPH tissue was performed at 47°C for 3 h, like clinical therapy. Whether thermal treatment can induce changes in the secondary structure of epithelium or stroma in BPH tissue was investigated. The results indicate that the epithelium and stroma in BPH tissue had different protein secondary structures, due to the different compositions of the epithelium and stroma. No significant change was evidenced in secondary structure for each sample either before or after isothermal study, suggesting the integrality and safety of BPH thermotherapy in a 47°C for 3 h treatment course.