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Abstract
Chemical forms of selenium were studied in a solution extracted from soil with water or sodium sulfate solution by means of gel chromatography. When the soil extracts were chromatographed on a Sephadex G-25 column, the selenium in the extracts was separated into high and low molecular-weight fractions. A large proportion of the selenium in both fractions occurred in an organically-bound form and a small amount of inorganic selenium was found in the low molecular-weight fraction. The distribution of selenium on the chromatogram agreed well with that of fluorescamine-reactive substances. It was considered that the selenium in the soil extracts was mainly contained in proteins, peptides or amino acids. In addition, the high molecular-weight fraction containing selenium extracted from an Andosol was found to be a polypeptide with a molecular-weight of about 3,200 and was assumed to be composed of about 29 amino acids.
