Preparation of antibody of mungbean leghemoglobin

Danilo M. Mendoza Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; National Institutes of Biotechnology and Applied Microbiology (BIOTECH), University of the Philippines at Los Banos, College, Laguna, Philippines

Praphasri Chongpraditnun Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; Department of Agriculture, Ministry of Agriculture and Cooperatives, Bangkhen, Bangkok, Thailand

Mitsuo Chino Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

Keiji Suzuki Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; School of Medicine, Gunma University, Maebashi, Japan

Shigenori Kurashige Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; School of Medicine, Gunma University, Maebashi, Japan

Abstract

A procedure for preparing the antibody of mungbean (Vigna radiata (L.) Wilzeck) leghemoglobin using synthetic oligopeptide is discussed. Results of the Western blot analysis revealed that the purified antibody crossreacted specifically with the leghemoglobins among the proteins extracted from mungbean root nodules.

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Preparation of antibody of mungbean leghemoglobin

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