![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
A new method, involving the application of PMR spectroscopy for the assay of piperazine anhydrous, hydrate and its salts is described. The PMR spectrum of piperazine in D2o has a well-defined singlet system for the piperazine ring protons which is chosen for the quantitative measurement. The principal of the method involves comparing the integral of this signal to that of the sharp singlet (positioned at 0.00 ppm) of trimethyl silyl propane sulfonate sodium salt which is used as PMR reference and internal standard.
The proposed method which is simple and rapid gives results of high degree of accuracy and percision when applied to the assay of pure piperazine anhydrous, hydrate and dihydrochloride samples. An average recovery percent of 100.24 ± 0.89 was obtained. The method was used for the analysis of piperazine hydrate in syrup and effervecent granules. The average recovery percent of the added piperazine was 99.64 ± 0.75. In addition the PMR spectrum obtained helps in confirming the identity and purity of the drug or its salts.
