Plumbagin, a Naturally Occurring Naphthoquinone: Its Isolation, Spectrophotometric Determination in Roots, Stems, and Leaves in Plumbago Europaea L.

Mohammed A. Al-Nuri Chemistry Department, An-Najah N. University, Nablus West Bank, Via Israel

Mohammed A. Hannoun Chemistry Department, An-Najah N. University, Nablus West Bank, Via Israel

Nidal A. Zatar Chemistry Department, An-Najah N. University, Nablus West Bank, Via Israel

Maher A. Abu-Eid Chemistry Department, An-Najah N. University, Nablus West Bank, Via Israel

Waheed J. Al-Jondi Chemistry Department, An-Najah N. University, Nablus West Bank, Via Israel

Ahmad I. Hussein Chemistry Department, An-Najah N. University, Nablus West Bank, Via Israel

Mohammed S. Ali-Shtayeh Biology Department, An-Najah N. University, Nablus West Bank, Via Israel

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Abstract

A new method for isolation and spectrophotometric determination of plumbagin is presented. Plumbagin was isolated by thin layer chromatography (TLC) and column chromatography (CC) techniques, as an orange tinged yellow long crystalline substances. Plumbagin exhibits two absrop-tion maxima at 410 and 510 nm. Stability of the color, pK_a value, and the effect of pH were studied. Beer's law is obeyed over the range 0.9-45 ppm. The method is applied to the determination of plumbagin in roots, stems, and leaves of Plumbago europaea L. plant.

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Plumbagin, a Naturally Occurring Naphthoquinone: Its Isolation, Spectrophotometric Determination in Roots, Stems, and Leaves in Plumbago Europaea L.

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Plumbagin, a Naturally Occurring Naphthoquinone: Its Isolation, Spectrophotometric Determination in Roots, Stems, and Leaves in Plumbago Europaea L.

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