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Abstract
Fourier transform infrared spectroscopy has been used to study quantitatively the acetylation of monodisperse polystyrene microspheres with diameters ranging from 7 to 9μm. The CH2 stretching infrared vibration mode at 2921 cm−1 was used as the internal intensity standard. The acetylation extent could be easily measured by comparing the relative intensities of the bands of acetyl group (1678, 1415, 1359 cm−1) or the bands due to the para-substituted benzene ring to the band at 2921 cm−1 from the calibration curve.