Sers Studies on a Novel Copper Porphyrin and Its Complex with DNA

Abstract

The SERS spectra of copper (II) complex of meso-tetris (4-N-cyano methyl pyridyl) porphyrin (Cu¹¹NACN) and the complexes with the calf thymus double-stranded and denatured DNA on silver sols obtained by reducing AgNO₃ with citrate and the effect of pH and the concentration of Cu¹¹NACN have been investigated. It was pointed out that, pH=5∼6, the concentration of Cu¹¹NACN ca 1 × 10⁻⁵ mol/L are better for SERS. Cu¹¹NACN acts on Ag sols through the pyridyl N and the porphyrin core together, and is absorbed on Ag sols in the slope-on form which is not influenced by adding the double stranded and the denatured DNA. In the system of Ag sols, Cu¹¹NACN acts on the double stranded DNA at phosphate by electrostatic force, but maybe part-intercalates the single stranded of the denatured DNA accompanied with the electrostatic force similar to the double stranded DNA.

Key Words:

• Copper (II) complex of meso-tetris (4-N-cyano methyl pyridyl) porphyrin
• SERS
• Double-stranded DNA
• Denatured DNA
• Silver sols