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In the search for chemical probes of DNA conformations, we report an efficient synthesis of new Tröger's Base analogs derived from polyfunctionalized aminoacridines treated with a stoechiometric amount of formaldehyde in trifluoroacetic acid. For the more sensitive aminoacridines, the Tröger's Bases were obtained by nucleophilic substitution of the chloro group of the “pre-formed” corresponding Tröger's Base.
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