Reagents for Multiple Non-Radioactive Labelling of Oligonucleotides

Vladimir A. Korshun Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871, Russia

Nikolai B. Pestov Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871, Russia

Elena V. Nozhevnikova Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871, Russia

Igor A. Prokhorenko Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871, Russia

Sergei V. Gontarev Belarussian Research Institute for Epidemiology & Microbiology, Belarussian Ministry of Health, ul. K. Zetkin 4, Minsk, 220050, Belarus

Yuri A. Berlin Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871, Russia

Abstract

Novel phosphoramidite reagents for nonradioactive polylabelling of oligonucleotides have been developed, including two branching reagents, 4 and 8, and a biotin-containing phosphoramidite 19 which can be used to incorporate a biotin moiety into any position of the oligonucleotide.

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Reagents for Multiple Non-Radioactive Labelling of Oligonucleotides

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Reagents for Multiple Non-Radioactive Labelling of Oligonucleotides

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