In-field whole genome sequencing using the MinION nanopore sequencer to detect the presence of high-prized military targets

ABSTRACT

As DNA sequencing technology advances, in-field genome sequencing capabilities are being explored, utilized and validated. With the assistance of such advances, forensic DNA processing is at the dawn of an era of mobile analyses, as a supplement to the ‘bricks and mortar’ lab model that it is current practice. The prospect of getting a preliminary DNA identification result quickly and in the field can assist in staying on the trail of military targets. This pilot study aimed to assess the capability of the MinION nanopore DNA sequencer in a mobile lab scenario, using as little intervention as possible to evaluate the data produced and the possible impact and interpretation that might arise from it. Results from this study may help guide the direction of further research using this device and relevant software and tools.

KEYWORDS:

• In-field
• mitochondrial DNA
• nanopore DNA sequencing
• SNP
• STR

Acknowledgements

Dr Stanley Pang for his assistance with bioinformatics and extracting the data files.

Disclosure statement

No potential conflict of interest was reported by the authors.