Adaptation of a complement fixation test for large scale serological diagnosis of bovine leptospirosis

Summary

Details are given of a method of performing a semi-automated complement fixation test for the diagnosis of bovine leptospirosis. A “Dynatiter” machine is used which enables 288 sera to be tested per hour. In a comparative study it was shown that using this semi-automated test fewer than 5% of antibody titres differed from those obtained using a manual test, either with single antigens or a polyvalent antigen.