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Abstract
Pig kidneys naturally infected with Leptospira interrogans serova pomona were subjected to chilling (0 ° C) and freezing storage (−15 ° C). Seven out of 9 (78%) kidneys still contained viable leptospires after 14 days at 0 ° C and 8 out of 10 (80%) after 30 days at −15 ° C. Both experimental chilling and freezing occurred at an average rate of l ° C/h. It is concluded that the commercial processes of chilling and freezing do not eliminate leptospires from infected pig kidneys.