17
Views
11
CrossRef citations to date
0
Altmetric
Scientific Article

Stability of glutathione peroxidase in ovine blood samples under various storage conditions and the response of this enzyme to different methods of selenium supplementation

&
Pages 77-80 | Published online: 23 Feb 2011
 

Abstract

Levels of the selenium-containing enzyme glutathione peroxidase (GSH-Px) in haemolysates of ovine whole blood and in washed erythrocytes were almost identical, indicating that plasma has no significant effect on the method used.

Blood containing sodium heparin, sodium EDTA or fluoride/oxalate as anticoagulant could be stored at 4° or 20° for at least a week without appreciable loss of GSH-Px activity. At 27°, storage time should not exceed 4 days. Irrespective of the anticoagulant used, GSH-Px levels in haemolysates prepared on the day of blood collection and stored at 4° remain unchanged over 7 days. At 20° or 27° storage times should be limited to 2-3 days in the case of blood containing sodium heparin or sodium EDTA and to 2 or 1 day at 20° or 27° respectively in the case of fluoride/oxalated blood.

In sheep treated orally or subcutaneously with sodium selenite or sodium selenate, maximum levels of GSH-Px in the blood were reached in approximately 30 days. Thereafter the GSH-Px levels fell only slowly throughout the 108 day experimental period.

Using the analytical method described, GSH-Px levels in ovine blood of ≤ 20 μmoles NADPH oxidised min-1.g Hb-1. indicate a selenium deficiency.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.