Restriction endonuclease (EcoR₁) analysis of Brucella ovis DNA

Abstract

Seven Brucella oris isolates from diverse sources (including two used in the manufacture of a Br. ovis vaccine, four field strains and a reference strain from the National Culture Type Collection in London) were subjected to bacterial restriction endonuclease DNA analysis using the endonuclease EcoR₁. No genetic differences were detected. It was concluded that strain variation is unlikely to be a problem in Br. ovis control schemes, whether these are based on vaccination or on the eradication of the disease using serological tests.