The efficacy of a subcutaneous injection of soluble Vitamirn B₁₂ in lambs

Abstract

Aims. To assess the efficacy of a soluble Vitamin B₁₂ injection in lambs by measuring changes in the serum and liver Vitamin B₁₂ concentrations.

Methods. Thirty-six lambs were injected subcutaneously with 2 mg of soluble Vitamin B₁₂ while another group of 36 served as untreated controls. Blood and liver biopsy samples for Vitamin B₁₂ determinations were collected just before the injection and at days 1, 2, 5, 8, 16, 24, 30 and 45.

Results. The serum Vitamin B₁₂ concentrations of the Vitamin B₁₂ treated lambs increased rapidly compared to the untreated lambs. Concentrations peaked at day 2, decreased rapidly to day 8, and then decreased more slowly until day 24 when there were no longer differences between the groups. Liver Vitamin B₁₂ concentrations of the Vitamin B₁₂ treated lambs were significantly greater over days 8-24.

Conclusion. A subcutaneous injection of 2 mg of soluble Vitamin B₁₂ was effective in increasing and maintaining the Vitamin B₁₂ status of lambs for about 24 days.

Clinical significance. This Vitamin B₁₂ product is only effective for preventing cobalt deficiency in lambs for about 4 weeks.

KEY WORDS:

• Vitamin B₁₂
• lambs
• Co deficiency
• serum
• liver