Causes of abortion on New Zealand dairy farms with a history of abortion associated with Neospora caninum

Abstract

AIM: To investigate the cause of abortion in cows from farms with a history of abortion associated with Neospora caninum.

METHODS: Cows and primiparous heifers (n = 2,246) that were enrolled in a clinical trial of a vaccine for N. caninum, and 403 cows and heifers that conceived >6 weeks after the planned start of mating (PSM), were monitored for abortion on five dairy farms with a history of abortion associated with N. caninum. When abortion was detected the fetus and/or placenta were submitted for histopathological examination and maternal paired sera were collected for the detection of antibodies to N. caninum using the indirect fluorescent antibody test (IFAT), bovine viral diarrhoea virus (BVDV) using ELISA, and Leptospira spp. using the microscopic agglutination test (MAT).

RESULTS: At the start of the clinical trial, 189/1,760 (10.7%) of cows and 61/486 (12.6%) of heifers had IFAT titres ≥1:200 for N. caninum. Abortions were detected in 111 (4.9%) enrolled and 37 (9.2%) non-enrolled cows and heifers. The relative risk of abortion was 4.21 (95% CI 2.92–6.08) times greater in enrolled animals that were seropositive to N. caninum than seronegative animals (p < 0.01). Among all animals, histopathological lesions suggestive of protozoal abortion were seen in nine, and of bacterial infection in 15, of the 40 cases where the fetus and/or placenta were recovered. IFAT titres for N. caninum ≥1:1,000 were detected in 66 cows at abortion, including 7/9 cases where there were fetal lesions indicative of N. caninum infection. In nine cows that aborted, there was evidence of seroconversion to BVDV and eight cows had MAT titres for Leptospira spp. ≥1:200. Histopathology revealed dual infectious aetiologies in two cases and, in another 17 cases, there was serological evidence of recent exposure to a second infectious agent capable of causing abortion in conjunction with N. caninum lesions in the fetus or fetal bacteraemia.

CONCLUSIONS: Multiple infectious aetiologies occur at herd-level and in individual cows on farms with a history of abortion associated with N. caninum. A diagnosis of abortion due to N. caninum infection was made in 9/34 (26.5%) cases where fetal histopathology was undertaken.

CLINICAL RELEVANCE: Thorough and ongoing diagnostic investigations should be undertaken in herds with higher than expected abortion losses to identify all causative agents as control measures could be implemented that may also reduce the risk of abortion in cows infected with N. caninum.

Key Words:

• Cattle
• abortion
• diagnosis
• Neospora caninum
• fetus

Acknowledgements

This study was funded by Intervet International B.V. (Boxmeer, The Netherlands) but the study was designed by the authors. Claire Nicholson, John Southworth and Mark Wyllie (Intervet, New Zealand) provided logistical and management support for the trial. Daniel Russell was responsible for the development and maintenance of the database used to record results. We thank Giles Gilling, Guy Oakley, Sandra van der Staay and Mark Bryan who were the local veterinarians for the farms that participated in this trial and who carried out much of the field work. Mark Collett (IVABS, Massey University) and Fraser Hill (Gribbles Veterinary Pathology, Palmerston North) provided advice on abortion diagnostics and Lachlan McIntyre (Ministry of Agriculture and Forestry, Biosecurity New Zealand) assisted with collation of national abortion diagnoses. Finally we offer our grateful thanks to the owners and managers of the farms that were involved in the trial.

Notes

*Non-peer-reviewed