Efficacy of inclusion of equine chorionic gonadotrophin into a treatment protocol for anoestrous dairy cows

Abstract

AIM: To compare the performance of intravaginal devices containing 1.0 g (DIB) or 1.38 g (CIDR) progesterone and to determine the efficacy of inclusion of equine chorionic gonadotrophin (eCG) into progesterone-based anoestrous cow treatment protocols for New Zealand dairy cows.

METHODS: Anoestrous cows (n = 1,906) from 12 herds were randomly assigned to four treatments: 100 μg gonadorelin (GnRH) at Day -10; 500 μg cloprostenol at Day -3; 100 μg GnRH at Day -1 and fixed time artificial insemination (FTAI) on Day 0 (gonadotrophin-prostaglandin-gonadotrophin [GPG] group, n = 475); GPG with CIDR device (1.38 g progesterone) inserted between Day -10 and Day -3 (CIDR group, n = 477); GPG with DIB device (1.0 g progesterone) inserted between Day -10 and Day -3 (DIB group, n = 477); and DIB with 400 IU eCG at Day -3 (DIB + eCG group, n = 477). Conception rates to FTAI and pregnancy at Day 28 were analysed using generalised estimating equations (GEE). Time to conception and time to return to oestrus were analysed using Kaplan-Meier survival analysis and Cox's proportional hazards regression.

RESULTS: The proportion of cows conceiving to FTAI was 0.34 (95%CI = 0.29–0.37), 0.38 (95%CI = 0.34–0.43), 0.38 (95%CI = 0.33–0.42) and 0.41 (95%CI = 0.37–0.46) for GPG, CIDR, DIB and DIB + eCG groups, respectively. The proportion of cows pregnant by Day 28 was 0.55 (95%CI = 0.51–0.60), 0.57 (95%CI = 0.52–0.61), 0.56 (95%CI = 0.52–0.60) and 0.63 (95%CI = 0.59–0.67) for GPG, CIDR, DIB and DIB + eCG groups, respectively. There was an interaction between treatment and number of days calved (p < 0.05). Cows more than 60 days calved and treated with DIB + eCG had higher FTAI conception and 28-day pregnancy rates than cows treated with GPG (p < 0.001). Median interval to conception did not differ between treatments (p > 0.05). There were no differences between DIB and CIDR groups for any parameter (p > 0.05). The range of the relative risk distribution among herds comparing DIB + eCG to DIB groups was greater than that comparing CIDR to DIB groups for conception to FTAI and pregnancy at Day 28.

CONCLUSIONS: The inclusion of eCG into progesterone-based anoestrous cow treatment protocols improves conception to FTAI and 28-day pregnancy rates in cows >60 days calved at treatment compared with a GPG protocol. There was no difference in clinical performance between DIB and CIDR devices.

CLINICAL RELEVANCE: The combination of a low payload (1.0 g) progesterone releasing intravaginal device with eCG treatment at device removal within a GPG treatment is a clinically effective treatment for anoestrous in New Zealand dairy cows.

KEY WORDS:

• dairy cattle
• anoestrous
• treatment
• eCG

Acknowledgements

The co-operation of the field veterinarians, herd owners and farm staff involved in this study is appreciated. The insightful recommendations by the reviewers have greatly improved this paper. AgriHealth New Zealand Limited funded this study with support from Syntex Argentina.